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目的建立一种基于毛细管电泳-化学发光联用法检测粮食中伏马菌素B1的快速检测方法。方法基于伏马菌素B1对鲁米诺-三价银[Ag(Ⅲ)]化学发光体系有抑制作用,结合毛细管电泳分离技术,对检测方法进行优化,选择5×10-3mol/L硼砂为电泳缓冲溶液,鲁米诺浓度为2×10-3mol/L,Ag(Ⅲ)浓度为3×10-5mol/L溶解于0.01 mol/L Na OH。结果经过条件优化后进行试验,伏马菌素B1的线性范围为1~200μg/ml,检出限(S/N=3)为1μg/ml。对200μg/ml的伏马菌素B1进行8次平行测定,其出峰时间和峰高的相对标准偏差分别为2.64%和7.86%。结论该方法操作简便、快速、准确可靠,可用于伏马菌素B1的检测,适用于玉米中伏马菌素B1的测定,结果比较理想。
OBJECTIVE To establish a rapid detection method for fumonisin B1 in food by capillary electrophoresis-chemiluminescence detection. The method was based on the inhibition of luminol-trivalent silver [Ag (Ⅲ)] chemiluminescence system based on fumonisin B1. Combined with capillary electrophoresis separation, the detection method was optimized. 5 × 10-3 mol / L borax Electrophoresis buffer solution, the luminol concentration of 2 × 10-3mol / L, Ag (Ⅲ) concentration of 3 × 10-5mol / L dissolved in 0.01mol / L NaOH. Results After the optimal conditions were tested, the linear range of fumonisin B1 was 1 ~ 200μg / ml, and the detection limit (S / N = 3) was 1μg / ml. Eight parallel determinations of 200 μg / ml fumonisin B1 showed relative standard deviations of 2.64% and 7.86%, respectively, in peak time and peak height. Conclusion The method is simple, rapid, accurate and reliable and can be used for the determination of fumonisin B1. It is suitable for the determination of fumonisin B1 in maize and the results are satisfactory.