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目的:建立高效毛细管电泳法测定麻杏石甘汤中的有效成分苦杏仁苷的含量。方法:采用内标法,选取芦丁为内标,未涂层熔融石英毛细管柱(67 cm×75μm,有效长度60 cm)为分离通道,以12 mmol·L~(-1)硼砂-乙醇(90:10)为电泳介质,分离电压25 kV,检测波长215 nm。结果:在上述条件下,苦杏仁苷在20 min内得到很好的分离,在0.31～6.21 mg·mL~(-1)与峰面积线性关系良好,平均加样回收率为98.4%。结论:方法简便快速、结果准确、重复性好,可用于麻杏石甘汤及其制剂的质量控制。
OBJECTIVE: To establish a high performance capillary electrophoresis method for the determination of amygdalin, the active ingredient in Maxingshigan Decoction. Methods: The internal standard method was used with rutin as the internal standard. The uncoated fused silica capillary column (67 cm × 75 μm, effective length 60 cm) was used as the separation channel. The extraction was performed with 12 mmol·L -1 borax-ethanol 90:10) as electrophoresis medium, separation voltage 25 kV, detection wavelength 215 nm. Results: Under the above conditions, amygdalin was well separated within 20 min. The linear range was 0.31 ~ 6.21 mg · mL ~ (-1) and the peak area was linear with the average recovery of 98.4%. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used for the quality control of Maxingshigan Decoction and its preparation.