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简述了微生物清防蜡原理及菌种筛选。所用菌种为已商品化的 4种兼性厌氧的烃氧化菌 ,生存温度 0～10 0℃ ,生长温度 2 0～ 6 0℃ ,最佳生长温度 30～ 4 5℃ ,4 0℃时在饶阳 3口井的原油中培养 4 0h ,菌数达到峰值 ,培养80h后菌数仍保持高值。将一种实验菌与 3口井的原油在 35～ 4 0℃培养 4 8h后 ,原油 4 5℃粘度降低 2 0 %～ 30 % ,凝固点降低 2～ 3℃ ,动、静态防蜡率达 93%～ 97%。介绍了微生物清防蜡的选井原则、工艺程序和方法。 10口试验井停止热洗和化学清防蜡 ,每隔 30d通过油套环空将 5 0～ 15 0kg菌液稀释后注入井筒泵下 ,油井功图正常 ,抽油机电流稳定中有所降低 ,有 5口井产油量略有增加。该技术经济上可行。
Briefly described the principles of microorganism anti-wax and strain screening. The bacterial strains used were 4 kinds of facultative anaerobic hydrocarbon oxidizing bacteria which were commercialized, with the survival temperature of 0 ~ 100 ℃, the growth temperature of 20 ~ 60 ℃, the optimum growth temperature of 30 ~ 45 ℃ and 40 ℃ In Raoyang 3 wells of crude oil culture 40h, the number of bacteria reached its peak, after 80h cultured bacteria remained high. After the crude oil from one kind of experimental bacteria and three wells was cultured at 35 ~ 40 ℃ for 48 h, the viscosity of crude oil decreased 20% ~ 30% at 45 ℃, and the freezing point decreased by 2 ~ 3 ℃. % ~ 97%. The selection principle, process procedure and method of microorganism paraffin removal are introduced. 10 test wells to stop the hot wash and chemical wax, every 30d through the oil ring annulus will be diluted 50 ~ 150kg bacterium into the wellbore pump, the well workplan normal, pumping current stability has been reduced , 5 wells produced a slight increase in oil production. This technique is economically viable.