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分枝数是大豆重要的农艺性状之一。对控制大豆分枝数的基因位点进行定位具有重要的理论和应用价值。本研究以寡分枝栽培大豆冀黄13为母本,多分枝地方品种小黑豆为父本配制杂交组合,分别在2012年以F2:3群体为定位群体利用寡分枝单尾法和在2014以F2:4群体为定位群体,利用双尾法选择性基因分型方法对大豆分枝数进行QTL定位研究。研究表明,2012年,在寡分枝单尾群体检测到一个L连锁群上BARC19-1222(71.32 c M)位点与分枝数QTL位点连锁,该位点与已经报道的q Br2和q BN24-1位点较近,可能为同一个位点;2014年,在F2:4分离群体中的双尾群体中共检测到2个与分枝数QTL位点连锁的位点,分别是F连锁群上的BARC13-1845位点和B2连锁群上的BARC14-1214位点。在其附近尚未有分枝数相关QTL位点的报道,这两个位点可能为新位点。本研究将为进一步进行分枝数QTL位点的精细定位和分子标记辅助选择育种奠定基础。
The number of branches is one of the important agronomic traits in soybeans. It has important theory and application value to locate the loci that control the number of branches of soybean. In this study, we used the oligotranched soybean Jhuanghuang 13 as the female parent and the multi-branch local hybrids Xiao-Hei-Dou as the male parent to make a cross combination. In 2012, Using F2: 4 population as the locating population, QTL mapping of soybean branching number was carried out by two-tail selective genotyping method. The results showed that in 2012, a locus of BARC19-1222 (71.32 c M) was detected in the single-tailed population of oligo-branch with linkage to QTL of branch number. This locus was similar to the reported q Br2 and q BN24-1 locus may be the same locus; in 2014, two loci linked to QTLs of branch number were detected in the two-tailed population of F2: 4 segregation population, namely F-linkage BARC13-1845 locus on the population and BARC14-1214 locus on the B2 linkage group. There are no reports of branch number-related QTLs in the vicinity of these sites, which may be new loci. This study will lay the foundation for further fine mapping QTL loci and molecular marker assisted selection breeding.