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目的探讨α-1,6岩藻糖基转移酶(α-1,6fucocyltransferase,FUT8)在不同前列腺癌细胞系、癌及癌旁组织中的表达差异及其意义。方法培养前列腺正常上皮细胞(RWPE-1)、4种前列腺癌细胞(22RV1、LNCap、PC-3、DU145)及2种其他类型泌尿系统肿瘤细胞(T-24、786-O),收集15对前列腺癌及癌旁组织,提取总RNA,采用RT-PCR检测不同细胞系、癌及癌旁组织中FUT8mRNA的表达,并进行差异比较。结果 FUT8 mRNA在前列腺癌细胞系中的表达高于前列腺正常上皮细胞系(RWPE-1)、膀胱癌细胞系(T-24)以及肾癌细胞系(786-O)。雄激素非依赖性前列腺癌细胞系(PC-3、DU145)中FUT8 mRNA的表达高于雄激素依赖性前列腺癌细胞系(LNCap);前列腺癌转移灶细胞系(PC-3、DU145)中FUT8mRNA的表达高于前列腺癌原发灶细胞系(22RV1)。同时还发现,FUT8mRNA在前列腺癌组织中的表达高于癌旁组织。以上差异均有统计学意义(P<0.01)。结论 FUT8在不同前列腺癌细胞系、癌及癌旁组织中存在差异表达,可能参与前列腺癌的雄激素非依赖性转化及进展转移等过程。
Objective To investigate the expression and significance of α-1,6 fucocyltransferase (FUT8) in different prostate cancer cell lines, carcinomas and paracancerous tissues. Methods RWPE-1, two kinds of prostate cancer cells (22RV1, LNCap, PC-3 and DU145) and two other types of urinary tumor cells (T-24 and 786-O) The expression of FUT8 mRNA in different cell lines, cancer and paracancerous tissues was detected by RT-PCR and the differences were compared. Results The expression of FUT8 mRNA in prostate cancer cell lines was higher than that of RWPE-1, T-24 and 786-O cell lines. The expression of FUT8 mRNA in androgen-independent prostate cancer cell lines (PC-3, DU145) is higher than in androgen-dependent prostate cancer cell line (LNCap); FUT8 mRNA expression in prostate cancer metastatic cell line (PC-3, DU145) Higher than the prostatic carcinoma cell line (22RV1). Also found that, FUT8 mRNA expression in prostate cancer tissue than paracancerous tissue. The above differences were statistically significant (P <0.01). Conclusions FUT8 is differentially expressed in different prostate cancer cell lines, carcinomas and paracancerous tissues, and may be involved in androgen-independent transformation and progression of prostate cancer.