恶性黑素瘤组织iNOS的表达与血管增生研究

• 作 者: 李娟 冯亚兰 吴东辉 徐晓芃 旷翎 冯浩
• 机 构: 北京市上地医院皮肤科,湖南省郴州市第一人民医院,湖南省人民医院,
• 出 处: 实用皮肤病学杂志
• 发表日期: 2015年05期
• 关键词: 恶性黑素瘤组织 恶性黑素瘤 色素痣 黑素瘤 恶性 恶性黑素瘤细胞 细胞增生核抗原 核抗原 血管生成 黑素瘤 免疫组化检测

目的探讨恶性黑素瘤组织中iNOS表达与增生及临床资料相关性。方法收集30例恶性黑素瘤患者皮损组织和20例色素痣患者的皮损组织,采用免疫组化检测恶性黑素瘤和色素痣组织中iNOS的表达,并与患者临床资料进行分析,同时观察其与癌细胞增生、血管生成的关系。结果 iNOS表达阳性率在恶性黑素瘤组织中比色素痣组织高(P<0.05),恶性黑素瘤iNOS表达在患者年龄、性别、部位中差异无统计学意义(P>0.05)。病理分期Ⅲ~Ⅳ期患者iNOS阳性率显著高于Ⅰ~Ⅱ期(P<0.05)。iNOS阳性肿瘤患者细胞增生核抗原(PCNA)标志指数(PLI)和微血管密度(MVD)的值明显高于iNOS阴性肿瘤患者(P<0.01)。结论 iNOS能促进恶性黑素瘤细胞增生与血管形成,是恶性黑素瘤的重要调节因子。

Objective To investigate the correlation between iNOS expression and proliferation in malignant melanoma and clinical data. Methods The lesions of 30 patients with malignant melanoma and 20 patients with pigmented nevus were collected. The expression of iNOS in malignant melanoma and pigmented nevus was detected by immunohistochemistry and analyzed with the clinical data of patients. To observe its relationship with cancer cell proliferation and angiogenesis. Results The positive rate of iNOS expression was higher in malignant melanoma than that in nevus (P <0.05). The expression of iNOS in malignant melanoma had no significant difference in age, sex and location (P> 0.05). The positive rate of iNOS in stage Ⅲ ~ Ⅳ patients was significantly higher than that in stage Ⅰ ~ Ⅱ (P <0.05). The values ​​of proliferating cell nuclear antigen (PCNA) index and microvessel density (MVD) in iNOS positive patients were significantly higher than those in iNOS negative patients (P <0.01). Conclusion iNOS can promote the proliferation and angiogenesis of malignant melanoma cells and is an important regulator of malignant melanoma.