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盐霉素是一种从白色链丝菌培养物中分离提取的聚醚类离子载体型抗生素,它广泛应用于畜禽类动物鸡球虫病的防治,并且还能作为饲料添加剂以促进畜禽的生长。最近新发现盐霉素具有特异性抑制肿瘤干细胞的作用。目前盐霉素钠的价格只是盐霉素的十分之一,但两者在抑制肿瘤细胞和肿瘤干细胞方面的异同却未见文献报导。本研究以人乳腺癌细胞MCF-7及其干细胞为模型,通过SRB实验对盐霉素及其钠盐的细胞毒性进行了评价和比较。首先,通过流式细胞仪从MCF-7细胞中分选得到了SP细胞;然后用乳腺癌干细胞表面特异性标志物CD44~+/CD24~-对SP细胞进行了鉴定;最后,分别测定了盐霉素和盐霉素钠对分选得到的肿瘤干细胞和肿瘤细胞的体外生长抑制率。结果表明,与乳腺癌细胞相比较,盐霉素和盐霉素钠对肿瘤干细胞均表现出了更强的抑制作用,在同样的给药浓度下,盐霉素和盐霉素钠对肿瘤细胞和肿瘤干细胞的抑制作用未表现出明显的差异。本研究结果提示,在抑制肿瘤干细胞的相关研究中,可以用盐霉素钠替代盐霉素而不会影响抑制效果。
Salinomycin is a polyether ionophilic antibiotic isolated from white streptococcal culture, which is widely used in the prevention and control of chicken coccidiosis in livestock and poultry and can also be used as a feed additive to promote livestock and poultry Growth. Recently, salinomycin has been newly found to inhibit tumor stem cells. At present, the price of sodium salinomycin is only one tenth of salinomycin, but the similarities and differences between the two in inhibiting tumor cells and tumor stem cells have not been reported in the literature. In this study, human breast cancer cells MCF-7 and stem cells as a model, by SRB experiments salinomycin and its sodium salt cytotoxicity were evaluated and compared. First, SP cells were sorted from MCF-7 cells by flow cytometry. Then SP cells were identified by CD44 ~ + / CD24 ~ - specific markers of breast cancer stem cells. Finally, In vitro and in vitro growth inhibition rate of selected tumor stem cells and tumor cells by mycotoxin and salinomycin sodium. The results showed that, compared with breast cancer cells, salinomycin and salinomycin sodium showed more inhibitory effect on tumor stem cells. Under the same administration concentration, salinomycin and salinomycin inhibited tumor stem cells Inhibition of tumor stem cells showed no significant difference. The results of this study suggest that salinomycin can be used to replace salinomycin without affecting the inhibitory effect in the related research of inhibiting tumor stem cells.