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[目的]构建毛竹萌发种子的全长cDNA文库。[方法]以毛竹萌发种子为材料,利用Oligo-capping法构建其全长cDNA文库。[结果]试验得到文库的库容达650万克隆,空载较少;插入片段大小在0.3~5.0kb之间,片段大小的分级严格且一致性良好,其中显著的是长片段全长cDNA(1.0~5.0kb)的比例高达30%,达到了高质量全长cDNA文库的标准。[结论]毛竹萌发种子全长cDNA文库的成功构建将为竹类植物功能基因组研究奠定重要的前期科研基础。
[Objective] To construct a full-length cDNA library of germinating seeds of bamboo. [Method] The germinating seeds of bamboo were used as materials to construct full-length cDNA library by Oligo-capping method. [Result] The library obtained had a capacity of 6.5 million clones with no load. The size of the inserted fragment was between 0.3-5.0 kb. The fragment size was strictly graded and the consistency was good. Among them, significant was the long fragment cDNA ~ 5.0kb) is up to 30%, reaching the standard of high quality full-length cDNA library. [Conclusion] The successful construction of the full-length cDNA library of germinating seeds of bamboo will lay an important pre-scientific basis for the functional genomics of bamboo plants.