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目的 :探讨糖基化终产物 (AGEs)对巨噬细胞一氧化氮通路的影响及其机制。方法 :应用一氧化氮 (NO)及一氧化氮合酶 (NOS)试剂盒测定巨噬细胞产生NO和NOS活性。结果 :巨噬细胞产生NO含量及NOS活性随AGEs作用时间延长明显减少 ,并且随AGEs的浓度、糖化程度加重、葡萄糖修饰浓度增加 ,巨噬细胞产生NO含量及NOS活性呈减弱趋势 ,VitE明显增加巨噬细胞产生NO含量以及NOS活性。结论 :AGEs通过抑制巨噬细胞NOS活性而抑制其产生NO ,VitE对巨噬细胞具有保护作用 ,能减轻AGEs的损害作用 ,这对糖尿病慢性并发症的防治提供了重要的理论依据
Objective: To investigate the effect of advanced glycation end products (AGEs) on nitric oxide (NO) pathway in macrophages and its mechanism. Methods: Nitric oxide (NO) and nitric oxide synthase (NOS) were used to determine the activity of NO and NOS in macrophages. Results: The content of NO and the activity of NOS in macrophages significantly decreased with the prolongation of AGEs. With the increase of AGEs concentration, the degree of saccharification and the concentration of glucose modified, the content of NO and the activity of NOS produced by macrophages tended to decrease, Macrophages produce NO and NOS activity. CONCLUSION: AGEs can inhibit NO production by inhibiting NOS activity of macrophages, and VitE has a protective effect on macrophages and can reduce the damage of AGEs, which provides an important theoretical basis for the prevention and treatment of chronic complications of diabetes