论文部分内容阅读
OBJECTIVE To investigate the relationship between the level of cas-pase-3 and 8 expression and intracellular Ca2+ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNа-treated groups and one control group as follows: an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca2+ concen-trations and the expression of caspase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-injection group. The expression of caspase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca2+ concentration. Caspase-3 and 8 expres-sion and the cellular Ca2+ level were higher following intratumoral injection compared to the other treatments (P<0.01). Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a) Both caspase-3 and 8 and the intracellular Ca2+ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tis-sues. These changes may play important roles in the occurrence and devel-opment of breast cancer; b) Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca2+ in apoptotic tumor cells.
OBJECTIVE To investigate the relationship between the level of cas-pase-3 and 8 expression and intracellular Ca2 + levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFN-treated groups and one control group as follows : an intratumoral injection, subcutaneous injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca2 + concen- trations and the expression of caspase-3 and 8 were examined. After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-injection group. The expression of caspase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca2 + concentration. Caspase-3 and 8 expres-sion and the cellular Ca2 + level were higher following intratumoral injection com Both of the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a) Both caspase-3 and 8 and the intracellular Ca2 + are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tis-sues. These changes may play important roles in the occurrence and devel-opment of breast cancer; b) Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca2 + in apoptotic tumor cells.