STS557诱变性研究

• 作 者: 王仁礼
• 出 处: 国外医学(计划生育分册)
• 发表日期: 1984年01期
• 关键词: 诱变性 事后避孕 共价结合 STS557 修复试验 雌烷 大分子物质 重组型 野生型 微粒体酶

有证据表明甾体激素可以与细胞内的大分子物质发生反应。如雌激素可与 DNA 共价结合,而孕激素在肝微粒体酶的代谢激化下可与蛋白质共价结合。作者对 STS 557(17α-Cyanomethyl-17β-hydroxy-estra-4,9-dien-3-one,即17α-氰甲基-17β-羟基-雌烷-4,9-二烯-3-酮、一种新的女用事后避孕药)的潜在诱变性研究中采用了下列测试系统:a)用变形杆菌 mirabilis PG273(野生型)和 PG713a(重组缺陷,her~-缺陷型)菌株的重组型修复试验测定 DNA 的初级损伤;

There is evidence that steroid hormones can react with intracellular macromolecules. For example, estrogen can be covalently bound to DNA, and progestins can be covalently bound to proteins by the metabolic activation of liver microsomes. The authors investigated the effect of STS 557 (17α-Cyanomethyl-17β-hydroxy-estra-4,9-dien-3-one, 17α-cyanomethyl-17β- A novel post-consumer contraceptive drug), the following test systems were used: a) recombinant strains of Proteus mirabilis PG273 (wild type) and PG713a (recombinant defective, her ~ -deficient) strains Repair test to determine the primary damage of DNA;