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目的用分子生物学原位杂交组化法研究视网膜生后发育过程中生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＯＭ）基因的表达。方法１０只ＳＤ大鼠分为５组，每组２只，年龄分别为１、２、３、４、８周。原位杂交探针为地高辛标记ＳＯＭ反义ｃＲＮＡ探针。结果１周时，ＳＯＭｍＲＮＡ杂交显色在神经母细胞层内侧及节细胞层，呈弱阳性；２周时，杂交信号逐渐增强，节细胞层呈中等强度染色，内核层为弱阳性；３～４周时，显色明显增加，节细胞层呈深紫色着色，而内核层仍为中等强度染色；８周时成年大鼠，节细胞层阳性细胞散在分布，染色较强，而内核层为弱阳性。结论大鼠出生后，视网膜内ＳＯＭ基因表达逐渐增加，于３～４周时达高峰，至成年时又下降，可能在视网膜生后发育过程中，对视网膜神经元的分化起重要作用。
Objective To study the expression of somatostatin (SOM) gene during retina development by molecular biology in situ hybridization (IHC). Methods Ten Sprague-Dawley rats were divided into 5 groups with 2 rats in each group. The rats were respectively 1,2,3,4,8 weeks old. In situ hybridization probe is a digoxigenin-labeled SOM antisense cRNA probe. Results At 1 week, hybridization of SOM mRNA was weakly positive on the medial side of neuroblastoma and on the stratum corneum. At 2 weeks, the hybridization signal was gradually enhanced, the stratum corneum cells were stained moderately, and the inner nuclear layer was weakly positive. At week 8, the coloration increased obviously, the deep purple coloration was observed in the cell layer, and the middle colony was still moderately stained. At 8 weeks, the positive cells in the ganglion cells were scattered and stained strongly, while the inner nuclear layer was weakly positive . Conclusion After birth, the expression of SOM gene in the retina gradually increases and reaches a peak at 3 to 4 weeks and declines to adulthood, which may play an important role in the retinal neuron differentiation during retina development.