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目的 :研究抗C2 1 5 单抗与超抗原———金黄色葡萄球菌肠毒素A(SEA)的融合蛋白C2 1 5 Fab SEA与C2 1 5 抗原基因转染的瘤苗共同激发的抗肿瘤效应。方法 :以脂质体法将人大肠癌相关C2 1 5 抗原的基因转染B16小鼠黑色素瘤细胞系制备成瘤苗 ,建立C5 7BL 6小鼠黑色素瘤荷瘤模型 ,观察其与融合蛋白C2 1 5 Fab SEA对肿瘤生长的抑制作用。结果 :融合蛋白与瘤苗联合治疗组小鼠的抑瘤率明显高于单用融合蛋白组和单用瘤苗组 (P<0 0 1)。结论 :融合蛋白C2 1 5 Fab SEA与C2 1 5 抗原基因转染的瘤苗 ,两者的抗肿瘤效应能相互增强 ,其共同激发的免疫应答能控制荷瘤动物肿瘤的生长。
OBJECTIVE: To study the anti-tumor effect of anti-C2155 monoclonal antibody and superantigen--Staphylococcal enterotoxin A (SEA) fusion protein C2 15 Fab SEA and C215 antigen gene transfected tumor vaccine . Methods: Tumor-bearing vaccines were prepared by transfecting human colorectal cancer-associated C215 antigen into B16 mouse melanoma cell lines by liposome method. The tumor model of melanoma in C57BL6 mice was established and observed with fusion protein C2. Inhibition of tumor growth by 15 Fab SEA. RESULTS: The tumor inhibition rate of the fusion protein and tumor vaccine-treated mice was significantly higher than that of the fusion protein alone group and the tumor vaccine group alone (P<0 01). CONCLUSION: The anti-tumor effects of the fusion protein C2 15 Fab SEA and C2 15 antigen can enhance each other’s anti-tumor effect. The co-excited immune response can control the tumor growth in tumor-bearing animals.