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目的探讨白花九里明提取液对急性肝损伤小鼠的保护作用及机制。方法取KM小鼠50只,随机均分为5组,正常对照组和模型对照组给予0.9%Na Cl注射液,联苯双酯组给予0.2 g/kg联苯双酯,白花九里明低浓度组和高浓度组分别给予15、30 g/kg的白花九里明提取液灌胃7 d;同时在灌胃的第1、3、5天,对正常对照组给予0.9%Na Cl注射液腹腔注射,模型对照组、联苯双酯组、白花九里明低浓度和高浓度组均给予0.1%四氯化碳(CCl4)溶液腹腔注射。末次灌胃1 h后,采血分离血清、取肝左叶制肝悬液分离上清液、取肝右叶制备组织切片。检测肝匀浆和血清的总蛋白(TP)含量、谷丙转氨酶(GPT)活性、谷草转氨酶(GOT)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,镜下观察肝组织结构变化。结果模型对照组、联苯双酯组、白花九里明低浓度组肝匀浆TP含量明显高于正常对照组(P均<0.01),白花九里明高浓度组肝匀浆TP含量明显高于正常对照组和模型对照组(P均<0.05),正常对照组、模型对照组、白花九里明低浓度组肝匀浆GPT活性明显高于联苯双酯组和白花九里明高浓度组(P均<0.05),白花九里明低浓度组血清GOT活性明显低于正常对照组(P<0.05);白花九里明高浓度组、联苯双酯组、模型对照组肝匀浆SOD活性明显低于正常对照组(P均<0.05),白花九里明低浓度组的肝匀浆SOD活性明显高于联苯双酯组(P<0.05),白花九里明低浓度组血清SOD活性明显高于正常对照组、模型对照组、联苯双酯组、白花九里明高浓度组(P均<0.01);白花九里明低浓度组和高浓度组肝匀浆MDA含量明显低于正常对照组(P均<0.05);其余各组血清TP含量、GPT活性、GOT活性、SOD活性、MDA含量组间两两比较差异无统计学意义(P均>0.05)。镜下示联苯双酯组、白花九里明低浓度组和高浓度组与模型对照组相比,肝组织结构清晰,肝细胞变性溶解坏死数量等均有较大改善,白花九里明低浓度组的少数中央静脉管腔内见少量的脱落肝组织结构。结论白花九里明对CCl4诱导的急性肝损伤有明显的保护作用,且呈浓度依赖性,高浓度白花九里明保护肝细胞效应与联苯双酯相当;其机制可能与促进和调动SOD清除自由基、阻断和降低肝细胞的脂质过氧化反应、提高肝细胞蛋白质合成有关。
Objective To investigate the protective effect and mechanism of Baihua Juli Extract on acute hepatic injury in mice. Methods Fifty KM mice were randomly divided into five groups. 0.9% Na Cl injection was given to the normal control group and model control group, and 0.2 g / kg bifendate was given to the bifendate group. Group and high concentration group were given 15, 30 g / kg of white Hedysarum extract for 7 days; the same time in the gavage of 1, 3, 5 days, the normal control group were given 0.9% NaCl injection intraperitoneal injection , Model control group, bifendate group, Baihua Jiuliming low concentration and high concentration group were given 0.1% carbon tetrachloride (CCl4) solution by intraperitoneal injection. After the last gavage 1 h, blood serum was separated, the liver suspension of the left lobe liver was used to separate the supernatant, and the right lobe of the liver was used to prepare tissue sections. The levels of total protein (TP), glutamic-pyruvic transaminase (GPT) activity, glutamic oxaloacetic transaminase (GOT) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) Liver tissue structure changes. Results The content of TP in the liver homogenate of model control group, bifendate group and Baihua Jiuliming low concentration group was significantly higher than that of the normal control group (all P <0.01). The content of TP in liver homogenate of Baihua Jiuli Ming high concentration group was obviously higher than that of normal control group (P <0.05). The GPT activity in liver homogenate of normal control group, model control group and Baihuadulimin low concentration group was significantly higher than that of bifendate group and Baihuaduliting high concentration group (P <0.01) <0.05). The serum GOT activity of Baihua Jiuli low concentration group was significantly lower than that of the normal control group (P <0.05). The SOD activity in liver homogenate of Baihua Jiuli Ming high concentration group, Bifendate group and model control group was significantly lower than normal (P <0.05). The activity of SOD in the liver homogenate of Baihua Jiu Luming low concentration group was significantly higher than that of the bifendate group (P <0.05), and the activity of SOD in serum of Baihua Jiuliming low concentration group was significantly higher than that in the normal control group , The model control group, the bifendate group and the Baihuaduli group (all P <0.01). The MDA content in the liver homogenate of the Baihua Jiu Liming low concentration group and the high concentration group was significantly lower than that of the normal control group (P <0.05) ); The other groups serum TP content, GPT activity, GOT activity, SOD activity, MDA content between the two groups was poor Not statistically significant (P> 0.05). Microscopically, Bifendate group, Baihua Jiuliming low concentration group and high concentration group compared with the model control group, the liver tissue structure is clear, the number of hepatocyte degeneration and necrosis were greatly improved, A small number of central venous lumen see a small amount of shedding of liver tissue structure. Conclusion Baihua Jiuli can protect CCl4-induced acute liver injury significantly and in a concentration-dependent manner. The high concentration of Baihuadulili protects hepatocytes from the effects of Biphenyl Dipeptide, which may be related to the promotion and mobilization of SOD to scavenge free radicals , Blocking and reducing the lipid peroxidation of liver cells, improve liver cell protein synthesis.