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大白菜霜霉肩菌[Peronospora para-sitica(pers.)Fr.]是一种专性寄生茵,不能离体培养,在相对湿度达85%以上,气温为16℃,光照正常的环境条件下,活体保存期仅3～4周.为此,作者探讨了大白菜霜霉病菌在低温条件下的保存技术,经接种试验有效保存期达到6个月.一、材料与方法1.供试品种昌乐雪头白菜、五莲四叶扣白菜、城阳青白菜.2.培育接种苗将供试种子直接播种于经消毒的营养钵内(每钵5株),待幼苗两
Peronospora para-sitica (pers.) Fr.] Is an obligate parasite and can not be cultivated in vitro. Under relative humidity of 85% and temperature of 16 ℃, under normal ambient light conditions , The life preservation period was only 3 ~ 4 weeks.Therefore, the authors discussed the preservation technology of Chinese cabbage downy mildew in cryogenic condition, and the effective storage period of inoculation test reached 6 months.Materials and Methods1. The tested varieties Changle snow cabbage, Wulian Siye buckle cabbage, city green cabbage .2. Breeding seedlings The seeds will be directly sown in the sterilized nutrition bowl (5 per bowl), until the seedlings two