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目的： 建立一种高效液相色谱快速测定人血清中头孢曲松浓度的分析方法。方法： 使用Ｂｉｏ－ Ｒａｄ 高效液相色谱仪， ＬｕｎａＴＭ Ｃ１８ （２） 柱（４－６ ｍ ｍ ×１５０ ｍ ｍ ， ５ μｍ） ， 流动相为甲醇－ ０－１ ％ 三乙胺水溶液（１５∶８５ ， 用磷酸调ｐＨ 至７－５） ， 检测波长为２４０ ｎｍ ， 内标为茶碱。结果： 头孢曲松人血清浓度在０－７５ ～３８４ μｇ·ｍ Ｌ－ １ 范围内线性良好， 最低检出浓度为０－５ μｇ·ｍ Ｌ－ １ ， 日内及日间精密度均优于５ ％ 。结论： 本方法经济、快速、灵敏、准确、专属性高。
Objective: To establish a method for the rapid determination of ceftriaxone in human serum by high performance liquid chromatography. Methods: Bio-Rad high performance liquid chromatography (LunaTM C18 (2) column (4-6 m × 150 m m, 5 μm) was used with the mobile phase consisted of methanol and 0-1% , PH adjusted to 7-5 with phosphoric acid), detection wavelength was 240 nm, the internal standard for theophylline. Results: The linearity of ceftriaxone in the range of 0-75 ~ 384 μg · m L -1 was linear with the lowest detectable concentration was 0-5 μg · m L -1. The intra- and inter-day precision was better than 5 %. Conclusion: The method is economical, fast, sensitive, accurate and specific.