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本文建立了提取与测定大鼠腹腔巨噬细胞（ＰＭφ）生物合成白三烯Ｂ_４（ＬＴＢ_４）及５－羟基二十碳四烯酸（５－ＨＥＴＥ）的高效液相色谱法，并研究了４个单味中药抗炎作用与对ＰＭφ产生ＬＴＢ_４及５－ＨＥＴＥ的影响。结果表明大黄、厚朴、白头翁及败酱草提取物对大鼠ＰＭφ合成以上二种介质均有明显抑制作用。对炎性介质ＬＴＢ_４产生的抑制率（％）分别为：大黄素（终浓度取５．０×１０~（－６）ｍｏｌ／Ｌ）为８８．０％；厚朴为９９．８％；白头翁为９４．９％及败酱草为５７．９％（以上终浓度均取５．９×１０~（－２）ｇ／Ｌ）。
In this paper, a high performance liquid chromatography method for the extraction and determination of rat peritoneal macrophage (PMφ) biosynthetic leukotriene B_4 (LTB_4) and 5-hydroxyeicosatetraenoic acid (5-HETE) was established and studied. The anti-inflammatory effects of single Chinese herbs and their effects on PMΦ production of LTB_4 and 5-HETE. The results showed that extracts of Rhubarb, Magnolia officinalis, Pulsatilla chinensis, and Patriniae extract had significant inhibitory effects on the two media of PMφ synthesis in rats. The inhibition rate (%) of inflammatory mediators LTB_4 was 88.0% for emodin (final concentration 5.0×10 −6 mol/L), and 99.8% for Magnolia; Pulsatilla It was 94.9% and 57.9% of P. edulis (above the final concentration was 5.9×10-2 g/L).