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通过对60例孕中期血清学酶联免疫法检测乙型肝炎病毒（HBV）阳性者。产后2-3天取母血、初乳应用套式聚合酶键反应（nestedPCR），进行HBV－DNA检测、阳性率分别为：22例HBsAg／anti-HBC／HBeAg阳性（称大三阳）母血占63．64％．初乳占40．91％，10例HBsAg／anti-HBc／anti-HBe阳性（称小三阳）母血占40．0％，初乳占30．0％，6例anti-HBs／anti-HBe阳性母血及初乳均为0．12例HBsAg阳性母血占16．7％，初乳占8．33％。母血HBV－DNA检出率均高于初乳。对HBsAg，尤其是anti-HBc和HBeAg均阳性的母亲，母乳喂养问题受到高度重视。
Sixty cases of hepatitis B virus (HBV) positive were detected by the second trimester serological enzyme-linked immunosorbent assay. Maternal blood and colostrum were taken 2-3 days postpartum, and nested PCR was used to detect HBV-DNA. The positive rates were 22 cases of HBsAg / anti-HBC / HBeAg positive Blood accounted for 63.64%. Colostrum accounted for 40.91%, 10 cases of HBsAg / anti-HBc / anti-HBe positive (known as three positive) maternal blood accounted for 40.0%, colostrum accounted for 30.0%, 6 cases of anti-HBs / anti-HBe Positive maternal blood and colostrum were 0.12 cases of HBsAg positive maternal blood accounted for 16.7%, colostrum accounted for 8.33%. The detection rate of maternal HBV-DNA was higher than that of colostrum. Breastfeeding is highly valued for mothers who are positive for HBsAg, especially anti-HBc and HBeAg.