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在制作食用菌试管母种时,常因转管操作不慎而污染上好气性芽孢杆菌,有时从外地购回的试管种也常因破细菌污染而弃之。为此我们参考了有关抑制细菌生长的资料,经多次试验找到一种纯化的好方法,现简介如下:将配制好的PDA 培养基,装入三角瓶中,在1公斤/厘米~2高压下灭菌30分钟,待冷至50℃时,事先用无菌水配好的链霉素溶液,浓度为100毫升培养基古链霉素2000单位,充分混匀后注入培养皿内。冷凝
In the production of edible fungus tube mother, often due to improper tube transfer contamination of aerobic Bacillus, sometimes purchased from overseas test tube species often broken due to bacterial contamination and discarded. To this end, we refer to the information on the inhibition of bacterial growth, after several trials to find a good method of purification, are as follows: The prepared PDA medium, filled into a triangular flask, 1 kg / cm ~ 2 high pressure Sterilize for 30 minutes, until cold to 50 ℃, prior to use sterile water with streptomycin solution, the concentration of 100 ml medium streptomycin 2000 units, after mixing well into the Petri dish. Condensation