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目的探索3.0T MR活体示踪移植SPIO标记骨髓间充质干细胞(BMSCs)在大鼠脊髓损伤(SCI)模型中的优选序列。方法采用贴壁法培养大鼠BMSCs,使用超低微浓度的菲立磁-硫酸鱼精蛋白(Fe-Pro)标记BMSCs,普鲁士蓝及核固红染色显示细胞内铁。将20只脊髓损伤(SCI)模型的SD大鼠,于建模后第7天在损伤区两侧局部多点注射Fe-Pro标记的BMSCs,并于移植后第14天行3.0T MR FSET2WI、3DFIESTA、ESWAN序列成像,测量损伤区标记干细胞信号变化率及图像信噪比,并与脊髓组织切片普鲁士蓝染色对照。结果 20只SD大鼠BMSCs标记率为100%。脊髓普鲁士蓝染色显示,损伤区附近细胞质内大量细小蓝染颗粒。标记干细胞在3种MR序列图像上的信号变化率分别为(43.40±3.20)%、(77.80±3.59)%、(79.60±3.58)%,组间比较差异有统计学意义(P<0.05),FSET2WI序列的信号变化率均小于3DFIESTA及ESWAN序列的信号变化率,差异有统计学意义(P<0.05);3DFIESTA及ESWAN序列间信号变化率比较差异无统计学意义(P>0.05)。标记干细胞在3种MR序列图像信噪比分别为(20.95±1.91)、(45.70±9.40)、(44.50±8.29),组间比较差异有统计学意义(P<0.05),FSET2WI序列的信噪比均小于3DFIESTA及ESWAN序列的信噪比,差异有统计学意义(P>0.05);3DFIESTA及ESWAN序列间信噪比比较差异无统计学意义(P>0.05)。结论 3.0 T MR FSET2WI、3DFIESTA、ESWAN序列均可以检测到SPIO标记的BMSCs在大鼠脊髓内的MR信号,ESWAN序列效果最佳。
Objective To explore the optimal sequence of SPT labeled bone marrow mesenchymal stem cells (BMSCs) labeled with 3.0T MR in vivo in a rat model of spinal cord injury (SCI). Methods Rat BMSCs were cultured by adherent method. BMSCs were labeled with Fe-Pro ultra-low concentration, Prussian blue and nuclear red staining showed intracellular iron. Sprague Dawley (SD) rats with spinal cord injury (SCI) model were injected with Fe-Pro-labeled BMSCs on the 7th day after modeling. 3.0T MR FSET2WI was injected on the 14th day after transplantation. 3DFIESTA, ESWAN sequence imaging, the rate of change of signal of damaged stem cells and signal-to-noise ratio were measured, and compared with Prussian blue staining of spinal cord. Results The labeling rate of BMSCs in 20 SD rats was 100%. Prussian blue staining of the spinal cord showed a large number of small blue-stained particles in the cytoplasm near the injury area. The signal change rates of labeled stem cells in the three kinds of MR images were (43.40 ± 3.20)%, (77.80 ± 3.59)% and (79.60 ± 3.58)%, respectively. There was significant difference between the two groups (P <0.05) The rate of change of signal of FSET2WI was less than that of 3DFIESTA and ESWAN (P <0.05). There was no significant difference in the rate of signal change between 3DFIESTA and ESWAN (P> 0.05). The signal-to-noise ratios of labeled stem cells in the three MR sequences were (20.95 ± 1.91), (45.70 ± 9.40) and (44.50 ± 8.29), respectively. There was significant difference between the two groups (P <0.05) (P> 0.05). There was no significant difference in SNR between 3DFIESTA and ESWAN sequences (P> 0.05) .3. Conclusion MR signals of SPIO-labeled BMSCs in rat spinal cord can be detected by 3.0 T MR FSET2WI, 3DFIESTA and ESWAN sequences. ESWAN sequence has the best effect.