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目的 :探讨p53基因突变与大肠癌临床病理特点的关系。方法 :应用PCR SSCP技术检测 135例大肠癌患者p53基因第 5、6、7、8外显子的突变。结果 :153例大肠癌患者p53基因的突变率为54 0 7% ,p53基因突变与大肠癌的病理分级、临床分期、淋巴结转移及年龄均有关。结论 :p53基因与大肠癌的发生、发展及转归均有明显的关系。检测p53基因突变既可作为大肠癌的诊断方法 ,也可作为大肠癌预后的判定指标。
Objective: To investigate the relationship between p53 gene mutation and clinicopathological features of colorectal cancer. Methods: The mutations of exons 5, 6, 7 and 8 of p53 gene in 135 patients with colorectal cancer were detected by PCR SSCP. Results: The p53 gene mutation rate was 54 0 7% in 153 cases of colorectal cancer. The mutation of p53 gene was associated with the pathological grade, clinical stage, lymph node metastasis and age of colorectal cancer. Conclusion: The p53 gene has a clear relationship with the occurrence, development and prognosis of colorectal cancer. Detection of p53 gene mutation can be used as a diagnostic method of colorectal cancer, but also as a prognostic indicator of colorectal cancer.