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目的：研究利钾尿肽及心钠素前体分子内调控作用对心肌Ｎａ＋Ｋ＋ＡＴＰ酶的作用。方法：将大鼠心肌匀浆后，分别加入利钾尿肽、心钠素以及利钾尿肽＋心钠素，用比色法测定Ｎａ＋Ｋ＋ＡＴＰ酶活性。将大鼠心脏悬挂于Ｌａｎｇｅｎｄｏｒｆ灌流装置，分别以利钾尿肽、心钠素、利钾尿肽＋心钠素为灌流液，灌注心脏，用四道生理仪观测左心室内压、左心室收缩最大速率，左心室舒张最大速率，心率及冠脉流量。结果：心钠素虽然对Ｎａ＋Ｋ＋ＡＴＰ酶有抑制作用（抑制率２６．２％），但是，与对照无显著性差异（Ｐ＞０．０５）。利钾尿肽显著抑制酶的活性（抑制率４６．５％，Ｐ＜０．０１）这种抑制作用可被心钠素抵消（抑制率１７．６％，Ｐ＞０．０５）。利钾尿肽可以增加左心室收缩和舒张最大速率以及左室内压，而这种强心作用可因心钠素的加入而消失或减弱。结论：利钾尿肽可以抑制心肌Ｎａ＋Ｋ＋ＡＴＰ酶的活性，产生强心作用，心钠素可以抵消以上作用。
OBJECTIVE: To study the effect of kallikrein and atrial natriuretic peptide on the regulation of Na + K + ATPase activity in myocardium. Methods: The myocardial homogenates of rats were respectively added with kaliuretic peptide, atrial natriuretic peptide and kaliuretic peptide + atrial natriuretic peptide, and the activity of Na + K + ATPase was determined by colorimetry. The rat heart was suspended in a Langendorf perfusion device, and the heart was infused with Kaliuretic peptide, atrial natriuretic peptide and kaliuretic peptide + atrial natriuretic peptide, respectively. Left ventricular pressure Maximum rate, maximum rate of left ventricular diastole, heart rate and coronary flow. Results: Although the atrial natriuretic peptide has an inhibitory effect on Na + -K + -ATPase (26.2% inhibition rate), it has no significant difference with the control (P> 0.05). Kaliuretic peptide significantly inhibited enzyme activity (46.5% inhibition, P <0.01). This inhibitory effect can be offset by atrial natriuretic peptide (inhibition rate 17.6%, P> 0.05). Lee potassium-can increase the maximum left ventricular systolic and diastolic velocity and left ventricular pressure, and this cardiac effect may be due to the addition of atrial natriuretic peptide disappeared or weakened. Conclusion: KKP can inhibit the activity of myocardial Na + K + ATPase, resulting in a cardiac effect, atrial natriuretic peptide can counteract the above effect.