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目的:观察维甲酸对人神经母细胞瘤SK-N-SH细胞形态与超微结构及其相关标志物表达的影响,以鉴定其对神经母细胞瘤细胞终末分化的诱导作用。方法:1μmol/L维甲酸处理SK-N-SH细胞,光镜、电镜和免疫细胞化学检测研究SK-N-SH细胞处理前后细胞形态、超微结构变化和神经元相关标志物的表达变化。结果:光镜与电镜观察结果显示,SK-N-SH细胞经1μmol/LRA处理后,细胞形态和超微结构产生了细胞呈极性状、伸出多个轴突树突状突起、细胞逐渐变小变圆并融合在一起形成类似神经节样结构、细胞表面微绒毛减少、核仁变少变小、常染色质增多、细胞器丰富发达等显著变化;免疫细胞化学检测显示经RA处理后SK-N-SH细胞NSE、MAP2、Synaptophysin的表达较对照组细胞明显加强。结论:维甲酸能改变SK-N-SH细胞形态和超微结构恶性表型特征,并促进与神经细胞相关的终末分化指标的表达,从而对人神经母细胞瘤细胞的终未分化具有显著的诱导作用。
OBJECTIVE: To observe the effect of retinoic acid on the morphology and ultrastructure of human neuroblastoma SK-N-SH cells and the expression of related markers so as to identify the induction effect of retinoic acid on the terminal differentiation of neuroblastoma cells. Methods: SK-N-SH cells were treated with 1 μmol / L retinoic acid. The morphological changes, ultrastructural changes and expression of neuron related markers of SK-N-SH cells were detected by light microscope, electron microscope and immunocytochemistry. Results: The results of light microscope and electron microscope showed that the morphology and ultrastructure of SK-N-SH cells treated with 1μmol / LRA resulted in a polar cell shape with multiple axonal dendrites protruding and the cells gradually changing Small round and fused together to form similar ganglion-like structure, reduced microvilli on the cell surface, less and less nucleolus, increased euchromatin, rich organelles and other significant changes; immunocytochemistry showed that RA-treated SK- The expression of NSE, MAP2 and Synaptophysin in N-SH cells was significantly higher than that in control cells. CONCLUSION: Retinoic acid can change the morphological and ultrastructural features of SK-N-SH cells and promote the expression of terminal differentiation index related to nerve cells, which is significant for the terminal undifferentiated human neuroblastoma cells Induction.