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２０例临床诊断为散发性病毒性脑炎的尸检和活检脑组织，通过单纯疱疹病毒多克隆抗体免疫组化和生物素标记单纯疱疹病毒ＤＮＡ探针原位杂交研究，５例诊断为单纯疱疹病毒脑炎。结果说明：单纯疱疹病毒是国内散发性病毒性脑炎的重要病原；免疫组化和原位杂交是诊断单纯疱疹脑炎的两种敏感方法。
Twenty cases of autopsy and biopsy brain were randomly divided into three groups: autopsy and biopsy of sporadic viral encephalitis, immunohistochemistry of herpes simplex virus polyclonal antibody and in situ hybridization of biotin-labeled herpes simplex virus DNA probe, 5 cases were diagnosed as herpes simplex virus encephalitis. The result shows that herpes simplex virus is an important pathogen of sporadic viral encephalitis in China. Immunohistochemistry and in situ hybridization are two sensitive methods for the diagnosis of herpes simplex encephalitis.