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AIM:To clarify the protective effect of exogenous adenosinetriphosphate(ATP)on hypothermically preserved rat livers.METHODS:Establishment of continuous hypothermicmachine perfusion model,detection of nucleotides inhepatocytes with HPLC,measurement of activities of LDHand AST in the perfusate,observation of histopathologicalchanges in different experiment groups,and autoradiographywere carried out to reveal the underlying mechanism of theprotective effect of ATP.RESULTS:The intracellular levels of ATP and EC decreasedrapidly after hypothermic preservation in control group,whilea higher ATP and EC level,and a slower decreasing ratewere observed when ATP-MgCl_2 was added to the perfusate(P<0.01).As compared with the control group,the activitiesof LDH and AST in the ATP-MgCl_2 group were lower(P<0.05).Furthermore,more severe hepatocyte damage and neutrophilinfiltration were observed in the control group.Radioactive[α-~(32)P]ATP entered the hypothermically preserved rathepatocytes.CONCLUSION:Exogenous ATP has a protective effect onrat livers during hypothermical preservation.However,Mg~(2+)is indispensable,addition of ATP alone produces no protectiveeffect.The underlying mechanism may be that exogenousATP enters the hypothermically preserved rat liver cells.
AIM: To clarify the protective effect of exogenous adenosine triphosphate (ATP) on hypothermically preserved rat livers. METHODS: Establishment of continuous hypothermic macchine perfusion model, detection of nucleotides in hepatocytes with HPLC, measurement of activities of LDH and AST in the perfusate, observation of histopathological changes in different experiment groups, and autoradiography were carried to reveal the underlying mechanism of the protective effect of ATP .RESULTS: The intracellular levels of ATP and EC decreasedrapidly after hypothermic preservation in control group, whilea higher ATP and EC level, and a slower decrease ratewere viewed when ATP As compared with the control group, the activities of LDH and AST in the ATP-MgCl 2 group were lower (P <0.05) .Furthermore, more severe hepatocyte damage and neutrophilinfiltration were observed in the control group. Radioactive [alpha- (32) P] ATP entered the hypothermically preserved rathepatocytes. CONCLUSI ON: Exogenous ATP has a protective effect on coil during hypothermical preservation. However, Mg 2+ is indispensable, addition of ATP alone produces no protective effect. The underlying mechanism may be that exogenous ATP enters the hypothermically preserved rat liver cells.