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目的:探讨冬凌草乙素对U937细胞的生长抑制作用及其作用机制。方法:以不同浓度的冬凌草乙素作用于体外培养的U937细胞,MTT法检测细胞生长抑制率,应用hoechst33258荧光染色法观察细胞凋亡;采用RT-PCR对细胞凋亡前后β-catenin的表达水平进行检测。结果:冬凌草乙素可显著抑制细胞的生长及诱导细胞发生凋亡,呈现出明显的量-效与时-效关系。在冬凌草乙素抑制细胞增殖过程中β-catenin的表达水平显著下降。结论:冬凌草乙素能抑制U937细胞的生长并诱导细胞发生凋亡,降低β-catenin的表达水平可能是其重要作用机制之一。这为冬凌草乙素抗白血病的进一步研究提供了有力的实验依据。
Objective: To investigate the inhibitory effect of Rubescensine B on the growth of U937 cells and its mechanism. Methods: The cells of U937 cells were treated with different concentrations of Rubescens B and the cell growth inhibition rate was determined by MTT assay. The apoptosis of cells was observed by hoechst33258 fluorescence staining. The expressions of β-catenin Expression level for testing. Results: Rubescensine B could significantly inhibit cell growth and induce cell apoptosis, showing a significant dose-effect and time-effect relationship. The inhibitory effect of oridonin on the proliferation of β-catenin decreased significantly. Conclusion: Rubescensine B can inhibit the growth of U937 cells and induce cell apoptosis, and the decrease of β-catenin expression may be one of the important mechanisms. This provides a strong experimental evidence for the further study of Rubescensine B against leukemia.