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为确定巨大革耳种质资源间的亲缘关系,本文应用ISSR分子标记技术,对来源不同地区的野生或栽培的9个巨大革耳菌株进行遗传多样性分析。从20个引物筛选获得4个ISSR多态性引物对巨大革耳菌株扩增,获得23条多态性条带,多态性比率为85.19%;对扩增结果进行聚类分析,当遗传距离为20%时,9个菌株聚为2类:Ι类包括C.m0002菌株;Π类包括其它的8个菌株。其中C.m0002菌株与其它8个菌株的遗传距离很远。经栽培出菇实验,结果表明,C.m0002菌株的子实体似多脂鳞伞(黄伞),是同名异种;其它8个菌株均为巨大革耳。ISSR分析的结果与子实体形态特征一致。
In order to determine the genetic relationship between the germplasm resources of Giant Ligustrum, ISSR molecular markers were used to analyze the genetic diversity of 9 Giant Lode strains of wild or cultivated G. germans from different regions. Four ISSR polymorphic primers were screened from 20 primers to amplify the strains of Magnacum laevigata. Twenty-three polymorphic bands were obtained with a polymorphism ratio of 85.19%. Cluster analysis of the amplification results showed that when the genetic distance At 20%, nine strains were clustered into two groups: the class I consisted of C.m0002 strains; and the class Π included the other eight strains. Which C.m0002 strains and other 8 strains of genetic distance is very far. After cultivation and fruiting experiments, the results showed that the fruiting bodies of C.m0002 strain resembled multifamily (Umbrella), the same name and different species; the other eight strains were giant leather. The results of ISSR analysis are consistent with the morphological features of the fruiting bodies.