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目的观察酒精性肝病大鼠血清中白细胞介素18(IL-18)和肿瘤坏死因子α(TNF-α)水平的变化。方法大鼠随机分为:⑴实验组,用56%乙醇[5~9 g/(kg.d)]灌胃,共12周;(2)对照组,灌等容量生理盐水。实验第4,8,12周末分批处死动物。用HE染色观察肝脏病理学改变;用ELISA法检测血清中IL-18和TNF-α的浓度。结果实验组大鼠肝组织于4,8,12周时出现慢性酒精性肝病的各种表现,如脂肪变性、炎性改变及肝纤维化等。实验组血清ALT、AST含量高于对照组(P<0.05);实验组血清IL-18浓度明显降低而TNF-α明显升高,且其变化随病变程度的改变而变化。结论酒精性肝病大鼠血清细胞因子IL-18和TNF-α浓度的变化与病情严重程度有关,故可望作为酒精性肝病病情变化的动态指标。
Objective To observe the changes of serum levels of interleukin 18 (IL-18) and tumor necrosis factor α (TNF-α) in alcoholic liver disease rats. Methods The rats were randomly divided into: ⑴ experimental group, with 56% ethanol [5-9 g / (kg · d)] intragastrically for 12 weeks; (2) control group, irrigation volume of saline. Animals were sacrificed in batches on the 4th, 8th and 12th week of the experiment. Pathological changes of liver were observed by HE staining. Serum levels of IL-18 and TNF-α were detected by ELISA. Results The rats in the experimental group showed various manifestations of chronic alcoholic liver disease at 4, 8 and 12 weeks, such as steatosis, inflammatory changes and hepatic fibrosis. The level of serum ALT and AST in the experimental group was higher than that in the control group (P <0.05). The concentration of serum IL-18 in the experimental group was significantly decreased and the level of TNF-α was significantly increased. Conclusion The changes of serum cytokines IL-18 and TNF-α in alcoholic liver disease are related to the severity of the disease, so it is expected to be a dynamic indicator of the change of alcoholic liver disease.