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目的:观察上皮间质转化(epithelial-mesenchymal transition,EMT)相关因子E-钙黏着蛋白(E-cadherin)和波形蛋白(vimentin)以及干细胞因子八聚体结合转录因子4(octamer-binding transcription factor 4,OCT4)在浸润性乳腺癌中的表达情况及OCT4与E-钙黏着蛋白和波形蛋白的相关性。方法:收集河北医科大学第四医院2009年手术切除的浸润性乳腺癌组织标本40例,实时荧光定量PCR和免疫组织化学法分别检测OCT4、E-钙黏着蛋白和波形蛋白的mRNA和蛋白的表达,分析其与临床病理特征的关系、OCT4和EMT相关蛋白之间的相关性以及与浸润性乳腺癌患者生存率之间的关系。结果:在浸润性乳腺癌组织中,OCT4、E-钙黏着蛋白和波形蛋白表达率分别为30%(12/40)、55%(22/40)和65%(26/40)。OCT4表达与浸润性乳腺癌患者的年龄、组织学分级、淋巴结转移以及Her-2的表达有关,E-钙黏着蛋白表达与淋巴结转移有关,波形蛋白表达与组织学分级和淋巴结转移有关。OCT4 mRNA和蛋白的表达与E-钙黏着蛋白和mRNA蛋白的表达呈负相关,与波形蛋白mRNA和蛋白的表达呈正相关,而E-钙黏着蛋白mRNA和蛋白的表达与波形蛋白mRNA和蛋白的表达也呈负相关。OCT4表达阳性患者的生存率明显低于表达阴性患者,差异具有统计学意义(P<0.05);而E-钙黏着蛋白和波形蛋白的表达与患者生存率之间未见明显相关性(P>0.05)。结论:干细胞因子OCT4可能通过EMT促进浸润性乳腺癌细胞的转移,可能是影响浸润性乳腺癌预后的因素。
Objective: To observe the expression of E-cadherin and vimentin in epithelial-mesenchymal transition (EMT) and octamer-binding transcription factor 4 , OCT4) in invasive breast cancer and the correlation between OCT4 and E-cadherin and vimentin. Methods: Forty cases of invasive breast cancer specimens collected from 2009 Fourth Hospital of Hebei Medical University were collected. The mRNA and protein expressions of OCT4, E-cadherin and vimentin were detected by real-time fluorescence quantitative PCR and immunohistochemistry respectively , Analyzed its relationship with clinicopathological features, the correlation between OCT4 and EMT-related proteins and the relationship with the survival rate of patients with invasive breast cancer. Results: The expression rates of OCT4, E-cadherin and vimentin in invasive breast cancer were 30% (12/40), 55% (22/40) and 65% (26/40) respectively. OCT4 expression was correlated with age, histological grade, lymph node metastasis and Her-2 expression in invasive breast cancer. E-cadherin expression correlated with lymph node metastasis. The expression of vimentin was related to histological grade and lymph node metastasis. The expression of OCT4 mRNA and protein was negatively correlated with the expression of E-cadherin and mRNA, positively correlated with the expression of vimentin mRNA and protein, while the expression of E-cadherin mRNA and protein was positively correlated with the expression of vimentin mRNA and protein Expression is also negatively correlated. The survival rate of OCT4 positive patients was significantly lower than that of negative patients (P <0.05), while there was no significant correlation between E-cadherin and vimentin expression and patient survival (P> 0.05). Conclusion: The stem cell factor OCT4 may promote the metastasis of invasive breast cancer cells through EMT, which may be the factor that affects the prognosis of invasive breast cancer.