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目的：内抑素（Ｅｎｄｏｓｔａｔｉｎ）是一种有效的血管生成抑制因子，分离、克隆和测定中国人的Ｅｎｄｏｓｔａｔｉｎ基因，为进一步研究Ｅｎｄｏｃｔａｔｉｎ的功能奠定了基础。方法：从中国人胎肝组织中提取ｍＲＮＡ，用ＲＴ－ＰＣＲ方法将Ｅｎｄｏｓｔａｔｉｎ的ｃＤＮＡ扩增出，克隆到ｐＧＥＭ－Ｔ载体中，测定其ＤＮＡ序列。结果：中国人的Ｅｎｄｏｓｔａｔｉｎ ｃＤＮＡ序列被成功克隆，序列分析证实为 Ｅｎｄｏｓｔａｔｉｎ基因。结论：中国人的Ｅｎｄｏｓｔａｔｉｎ ｃＤＮＡ基因编码序列与国外文献报道的相应序列完全一致。
OBJECTIVE: Endostatin is a potent inhibitor of angiogenesis. The isolation, cloning and determination of Chinese Endostatin gene provide the basis for further study on the function of Endoctatin. Methods: mRNA was extracted from fetal liver tissue of Chinese. The cDNA of Endostatin was amplified by RT-PCR and cloned into pGEM-T vector. The DNA sequence was determined. Results: The Chinese Endostatin cDNA sequence was successfully cloned and sequence analysis confirmed Endostatin gene. Conclusion: The coding sequence of Endostatin cDNA in Chinese is exactly the same as that reported in foreign literature.