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研究影响大白菜、甘蓝和红菜薹小孢子胚状体再生成苗的几个生理因素的结果表明,在1.0%～1.2%的琼脂中胚状体再生成苗率显著高于0.8%琼脂的。4℃处理10d可显著提高大白菜和甘蓝胚状体再生成苗率。大白菜和红菜薹胚状体再生成苗的最适胚龄为20～29d,甘蓝则为30～35d。培养基B5和MS对再生成苗率影响不大。检测3种芸苔属蔬菜小孢子再生植株的倍性结果表明,大白菜和红菜薹小孢子植株自然加倍率较高,均超过70%;甘蓝较低,仅为30%左右。同一物种的不同品种间胚状体再生成苗所需的条件和加倍效率基本一致。
The results of several physiological factors that affect the regeneration of microspore embryoids from Chinese cabbage, cabbage and Chinese red cabbage showed that the regeneration rate of embryoid bodies in 1.0% -1.2% agar was significantly higher than that of 0.8% agar . Treatment at 4 ℃ for 10 days significantly increased the seedling regeneration rate of embryoid bodies of Chinese cabbage and cabbage. The optimum embryo age of regenerating embryos of Chinese cabbage and Chinese cabbage was 20 ~ 29 days, while that of cabbage was 30 ~ 35 days. Medium B5 and MS had little effect on the rate of regenerated seedlings. The results of ploidy detection of microspore regenerated plants of three Brassica vegetables showed that the microspore plants of Chinese cabbage and Chinese red rape blossus had a higher natural doubling rate, both of which exceeded 70%; the cabbage was low, only about 30%. The same species of different varieties of embryoid body regenerated seedlings required conditions and doubling efficiency are basically the same.