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大多数种类的动物卵巢内均有前列腺素(PG),其生成随卵巢功能状态而变化,除在卵巢破裂和某些种类动物LH对卵巢发挥调节作用时起重要作用外,对类固醇生成亦有显著作用。作者通过调节内源性前列腺环素(PGI_2)在卵巢各型细胞内的合成并研究其促进卵巢主要类固醇生成的影响,研究外源性PGI_2及其稳定的同类物对卵巢各种细胞内类固醇合成的作用,来探索PGI_2在卵巢类固醇生成中可能起的作用。实验采用未孕山羊的新鲜卵巢,用含抗生素的Hank氏液清洗卵巢4～5遍,以去除粘着组织和覆盖膜。再游离卵巢中直径为3～10mm排卵前卵泡的粒层细胞、卵泡膜细胞、黄体细胞从黄素化卵
Most species of animal ovaries have prostaglandins (PG), its generation with the ovarian function status changes, in addition to rupture of the ovary and some species of animal LH play an important role in the regulation of the ovary, but also on steroidogenesis Significant effect. By regulating the synthesis of endogenous prostacyclin (PGI_2) in various ovarian cells and studying its effects on ovarian steroidogenesis, we investigated the effects of exogenous PGI_2 and its stable congeners on various cellular intracellular steroids To explore the role of PGI_2 in ovarian steroidogenesis. In the experiment, fresh ovaries of non-pregnant goats were used. Ovaries were washed 4 to 5 times with Hank’s solution containing antibiotics to remove the adhesive tissues and the cover film. Then the free ovary in the diameter of 3 ~ 10mm preovulatory follicles granulosa cells, theca cells, luteal cells from luteinized eggs