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为阐明Ｏ１３９霍乱弧菌的主要分子生物学特征，并为探讨其来源提供科学依据，应用核酸脉冲场凝胶电泳、基因探针Ｓｏｕｔｈｅｒｎ杂交等方法，对国内外分离的２３株Ｏ１３９菌和一些Ｏ１群菌株进行分析。结果表明，所有Ｏ１３９菌株均表现为一致的且具有特征性的酶切图谱；它们与埃尔托型流行株的图谱较与古典型菌株图谱更为相似，与埃尔托非流行株和其他非Ｏ１菌株的遗传差异较大。在ＳｍａＩ图谱中，新疆分离的Ｏ１３９菌株显示出与当地１９８９年分离的埃尔托型流行株较接近的图谱；Ｓｏｕｔｈｅｒｎｂｌｏｔ结果显示在不同的酶切图谱分子杂交（ＳｍａＩ、ＳａｌＩ、ＸｂａＩｃｔｘ，ＢｇｌＩ１６ｓｒＲＮＡ）中，Ｏ１３９菌株与Ｏ１流行株之间有一定的差异；我国分离的Ｏ１３９菌株具有一致的图谱，与印度和孟加拉国分离的Ｏ１３９菌株之间有较小差异；新疆分离的Ｏ１３９菌株与该地１９８９年分离的某些ＥＶＣ流行株的图谱相似。基因检测表明，Ｏ１３９菌株都具有ｃｔｘ、ｚｏｔ、ａｃｅ和ＲＳ１；ＭＥＥ分析表明，Ｏ１３９菌株的ＥＴ型与ＥＶＣ流行株不可区分。
In order to elucidate the major molecular biological characteristics of Vibrio cholerae O139 and to provide a scientific basis for exploring its origin, 23 strains of O139 isolates isolated from China and abroad and some O1 genes were isolated by using nucleic acid pulsed-field gel electrophoresis and Southern blotting. Group of bacteria for analysis. The results showed that all of the O139 strains showed consistent and characteristic digestion maps; the maps of El Oto-type strains were more similar to those of the classical strains. Compared with Elto non-epidemic strains and other non-epidemic strains O1 strains of large genetic differences. In the SmaI map, the O139 strain isolated in Xinjiang showed a closer map to the El Tor type strain isolated in 1989 in the local area. Southernblot results showed that in different restriction enzyme maps (SmaI, SalI, XbaI -ctx, BglI 16srRNA), O139 strains and O1 epidemic strains have some differences between; O139 strains isolated in our country has a consistent map, with India and Bangladesh isolated O139 strains have little difference between; Xinjiang O139 strains isolated and The map of some EVC strains isolated in 1989 was similar. Genetic testing showed that all of O139 strains had ctx, zot, ace and RS1. MEE analysis showed that ET type of O139 strain was indistinguishable from that of EVC strain.