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目的 研究辐射和去雄激素对前列腺癌细胞凋亡通路基因表达的影响,探讨其协同诱导凋亡的机制。方法辐射与去雄激素作用前列腺癌细胞LNCaP,MTT实验和凋亡细胞染色分别评价细胞毒性和诱导凋亡作用。收集细胞提取总RNA并合成cDNA探针,在凋亡通路特异基因cDNA膜上进行杂交反应检测基因mRNA表达,并以RT-PCR确认有关基因mRNA表达。结果 辐射与去雄激素可协同诱导前列腺癌细胞凋亡。辐射使DFFA、LTbR、mdm2、Myd88、TNFRSF8Ⅱ、TNFRSF14和TNFSF4基因mRNA表达上调,使Survivin和Bar基因mRNA表达下调。去雄激素使Mcl-1、TNFRSF14、MyD88和TNFSF4基因mRNA表达上调,使Bar、Survivin和TRAIL-R3基因mRNA表达下调。结论 去雄激素和辐射对前列腺癌细胞凋亡通路基因的表达改变不同,这与两者协同诱导凋亡作用有关。
Objective To study the effects of radiation and androgen on the gene expression of apoptosis pathway in prostate cancer cells and to explore the mechanism of apoptosis induced by synergism. Methods Radiation and anti-androgenic effects Prostate cancer cells LNCaP, MTT assay and apoptotic cell staining were used to evaluate cytotoxicity and induce apoptosis respectively. The total RNA was collected and cDNA probes were synthesized. Hybridization was performed on the cDNA of apoptotic pathway-specific genes to detect the mRNA expression. RT-PCR was used to confirm the mRNA expression. Results Radiation and de-androgen can synergistically induce apoptosis of prostate cancer cells. Radiation induced up-regulation of mRNA expression of DFFA, LTbR, mdm2, Myd88, TNFRSF8II, TNFRSF14 and TNFSF4 genes, and down-regulated Survivin and Bar mRNA expression. Androgen deprivation up-regulates mRNA expression of Mcl-1, TNFRSF14, MyD88 and TNFSF4, and down-regulates mRNA expression of Bar, Survivin and TRAIL-R3. Conclusion Androgen and radiation have different effects on the expression of apoptotic pathway genes in prostate cancer cells, which is related to their synergistic effect in inducing apoptosis.