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目的:分析并探讨绒毛组织中抑癌基因PTEN和抗氧化蛋白PeroxiredoxinⅡ(Prx2)与自然流产的关系。方法:应用RT-PCR法及免疫组织化学SP法检测PTEN和Prx2蛋白及mRNA在30例自然流产患者(实验组)绒毛组织中的表达,并以同期非意愿妊娠要求人工流产的30例正常妊娠患者为对照组。结果:①实验组绒毛组织中PTEN和Prx2蛋白阳性表达率(70.0%,36.7%)明显低于对照组(93.3%,93.4%),差异有统计学意义(P<0.05)。②实验组绒毛组织中PTEN和Prx2 mRNA的表达(0.929±0.100,1.131±0.125)明显低于对照组(0.059±0.010,0.222±0.029),差异有统计学意义(P<0.05)。结论:PTEN和Prx2在自然流产患者绒毛组织中表达明显下降,说明可能与自然流产的发生有一定关系。
OBJECTIVE: To analyze and discuss the relationship between tumor suppressor gene PTEN and antioxidant protein Peroxiredoxin Ⅱ (voxx) and spontaneous abortion in villus tissues. Methods: The expression of PTEN and Prx2 protein and mRNA in 30 cases of spontaneous abortion patients (experimental group) were detected by RT-PCR and immunohistochemistry SP method, and 30 cases of normal pregnancy requiring abortion Patients as control group. Results: ① The positive rates of PTEN and Prx2 in the experimental group were significantly lower than those in the control group (70.0%, 36.7%) (93.3%, 93.4%), the difference was statistically significant (P <0.05). ② The expression of PTEN and Prx2 mRNA (0.929 ± 0.100,1.131 ± 0.125) in the experimental group was significantly lower than that in the control group (0.059 ± 0.010,0.222 ± 0.029), the difference was statistically significant (P <0.05). Conclusion: The expression of PTEN and Prx2 in villi of patients with spontaneous abortion decreased significantly, which may be related to the occurrence of spontaneous abortion.