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目的研究小鼠Osterix基因敲除及过表达后对其脊椎骨量的影响及作用机制。方法将12周龄的野生型小鼠(WT)、Osterix基因敲除小鼠(Osterix-KO)和Osterix过表达转基因小鼠(Osterix-CA)分为3组(每组8只,均为雄性),分别采用X线摄像和HE染色观察小鼠腰椎骨量的变化,TRAP染色检测破骨细胞水平,免疫组织化学检测NF-κB受体活化因子配体(RANKL)、骨涎蛋白(BSP)和骨桥蛋白(OPN)表达水平,并将所得数据进行统计学分析。结果 OsterixKO小鼠在X线和HE染色表现为腰椎骨密度和骨量增加,破骨细胞数量显著减少(P<0.05),椎体中RANKL、BSP和OPN的表达水平显著降低(P<0.05),而Osterix-CA小鼠的脊椎X线、HE染色表现及破骨细胞数量与WT小鼠无明显差异。结论 Osterix基因敲除小鼠脊柱骨吸收活性减弱伴成骨细胞分化能力降低,进而发生骨量明显增加。
Objective To investigate the effect of Osterix knockout and overexpression on the spine bone mass in mice and its mechanism. Methods Twelve-week-old wild type mice (WT), Osterix knockout mice (Osterix-KO) and Osterix overexpression transgenic mice (Osterix-CA) were divided into 3 groups (8 in each group, all male ). The changes of bone mass in lumbar vertebra were observed by X-ray and HE staining. The levels of osteoclasts were detected by TRAP staining. The expressions of RANKL, BSP, And osteopontin (OPN) expression levels, and the data were statistically analyzed. Results Osterix KO mice showed a significant increase in bone mineral density and bone mass in the lumbar spine (P <0.05). The expression of RANKL, BSP and OPN in vertebral bodies was significantly decreased (P <0.05) , While the X-ray, HE staining and osteoclast number of Osterix-CA mice showed no significant difference with WT mice. Conclusion Osterix knockout mice showed decreased bone resorption activity and reduced osteoblastic differentiation, which in turn increased bone mass.