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In acid medium, sparfloxacin is oxidized by nitrous acid. then reacts with hydrobromic acid to form a new fluorescence substance. which can emit the strong fluorescence. which is 151 fold more than that of sparfloxacin itself. By this . a new sensitive method for the determination of sparfloxacin in human urine by derivative-synchronous fluorescence is presented with good results.
Which can emit the strong fluorescence. Which is emitable strong to nitrous acid. Then reacts with hydrobromic acid to form a new fluorescence substance. determination of sparfloxacin in human urine by derivative-synchronous fluorescence is presented with good results.