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用RACE技术从受精后约22h的草鱼胚胎总RNA中扩增获得了草鱼MyoD基因的全序列,并对其序列进行了分析。结果表明,草鱼MyoD基因全长cDNA为1597bp,其中开放阅读框为825bp,共编码275个氨基酸,结构分析表明该肽链第1～84个氨基酸为草鱼MyoD基因的Basic区,第98～142个氨基酸为草鱼MyoD基因的HLH结构域,该序列所编码的肽链没有信号肽;通过对比分析已知的GeneBank中其它脊椎动物MyoD基因发现,该基因编码的氨基酸肽链随动物由低等向高等进化有加长的趋势,且核苷酸以及推测的氨基酸同源性和动物之间的亲缘关系相一致;草鱼MyoD基因的克隆为研究家鱼的肌肉发育调控的机理以及肉质改良奠定了基础。
The full - length sequence of MyoD gene was amplified by RACE from the total RNA of grass carp embryos about 22 h after fertilization. The sequence of MyoD gene was also analyzed. The results showed that the full-length cDNA of MyoD was 1597 bp in length and contained an open reading frame of 825 bp encoding a total of 275 amino acids. The structural analysis showed that the first to 84th amino acids of the grass carp were the Basic region of MyoD, The amino acid is the HLH domain of MyoD gene of grass carp. The sequence of the encoded polypeptide has no signal peptide. By comparing the MyoD gene of other vertebrates known in GeneBank, the amino acid peptide chain encoded by the gene is changed from low to high The evolutionary trend was lengthened, and the homology of nucleotide and deduced amino acid was consistent with that of animals. The cloning of MyoD gene in grass carp laid the foundation for studying the regulation mechanism of muscle development and meat quality in fish.