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本文应用流式细胞术(Flow Cytometry,FCM)检测了102例石蜡包埋的乳癌组织的细胞核DNA含量,并探讨了DNA指数(DNA Index.DI)和S期细胞比例(S-phase Fraction,SPF)与临床预后因素及病人术后生存状况的关系。结果显示:16例为DNA二倍体,8例近二倍体,78例(77％)非整倍体。非整倍体癌较二倍体/近二倍体癌的SPF高。DI值与组织学分级、淋巴结状况、原发肿瘤大小和临床分期密切相关,而与病人诊断时的年龄无关。淋巴结转移阳性组较阴性组、晚期肿瘤(Ⅲ、Ⅳ期)比早期肿瘤(Ⅰ、Ⅱ期)的DI值高(P<0.05),而非整倍体率仅有降低的趋势但无显著性差异,说明DI值作为一个预后参数比倍性分析统计效率高。SPF与病人的各预后因素及生存情况无明显关系。二倍体/近二倍体癌病人较非整倍体癌病人的生存率高、无瘤生存时间长,说明流式细胞术DNA含量测定确实可为乳癌病人的预后提供有价值的信息。
Flow Cytometry (FCM) was used to detect the nuclear DNA content of 102 paraffin-embedded breast cancer tissues, and DNA index (DI) and S-phase fraction (SPF) were discussed. ) Relationship with clinical prognostic factors and patient survival conditions. The results showed that 16 cases were DNA diploids, 8 cases were near diploids, and 78 cases (77%) were aneuploids. Aneuploid carcinoma has higher SPF than diploid/near diploid carcinoma. The DI value was closely related to histological grade, lymph node status, primary tumor size, and clinical stage, but not to the age at which the patient was diagnosed. The DI value of lymph node metastasis positive group was higher than that of negative group, advanced tumor (stages III and IV) compared with early stage tumors (stages I and II) (P<0.05), whereas the rate of non-euploidy was only decreased but there was no significant difference. The difference indicates that the DI value as a prognostic parameter is more efficient than the ploidy analysis statistic. There is no significant relationship between SPF and the patient’s prognostic factors and survival. Diploid/nearly diploid cancer patients have higher survival rate and longer survival time than non-euploid cancer patients, indicating that flow cytometry DNA content determination can indeed provide valuable information for the prognosis of breast cancer patients.