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将从大豆黑农16号分离的毒株SMV-H-16与美国阿肯色州分离的两个毒株SMV-S-6及S-7比较,三毒株的寄主范围基本相同,只系统侵染大豆,在几个菜豆品种上形成局部斑.H-16局部侵染昆诺藜,而S-6、S-7则不能.按Cho与Goodmon系统,H-16属G 5株系,S-6及S-7属于G 1株系.H-16及S-6提纯,在蔗糖梯度柱上只形成一条病毒区带.H-16电泳分析RNA及外壳蛋白质均只形成一条带,其分子量分别为2.9×10~6-3.2×10~6及26,000-26,500.分析超速离心只出现一个峰.沉降系数为135-138S.三株系与已知SMV抗血清作用生成沉淀线,彼此间不形成刺状物而完全融合.与WMV-2及WMV-E的抗血清呈阳性反应,而与TRSV、BPMV及WMV-1的抗血清呈阴性反应.粒体线状,长740mm左右.病叶组织中易见风轮状筒形内含物,并可见到病毒粒体.
Compared with the two isolates SMV-S-6 and S-7 isolated from Soybean Heinong 16 and S-7, the strains isolated from Soybean Heinong 16 had the same host range but only systemic infection Soybean, a local spot was formed on several kidney bean varieties.H-16 partially invaded quinoa, while S-6 and S-7 could not.According to Cho and Goodmon system, H-16 genotype G 5, S- 6 and S-7 belonged to the G 1 strain.H-16 and S-6 were purified to form only one virus band on the sucrose gradient column.H-16 electrophoresis analysis showed that only one band was formed between the RNA and the coat protein, 2.9 × 10 ~ 6-3.2 × 10 ~ 6 and 26,000-26,500. There was only one peak in the analysis of ultracentrifugation with a sedimentation coefficient of 135-138 S. The three lines generated precipitation lines with known antisera against SMV and did not form one another Thorn and completely fused with antisera of WMV-2 and WMV-E positive reaction, but negative reaction with TRSV, BPMV and anti-serum of WMV-1.Carticle linear, about 740mm long. It is easy to see the wind wheel-shaped cylindrical inclusions, and can see the virus particles.