Microcalorimetric Study on the Antibiotic Activity of Clarithromycin and Erythromycin

来源 :Wuhan University Journal of Natural Sciences | 被引量 : 0次 | 上传用户:harvy_chen
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By using LKB 2277 Bioactivity Monitoring System, the heat effect changes in the process of inhibitory action of clarithromycin and erythromycin on Escherichia coli at 37℃ were determined. Quantitativeanalysis showed that relationship between antibiotic concentration c and rate constant k of Escherichia coli growth, and half inhibitory ratio concentration IC 50 :clarithromycin: k = 0. 03003 1.1736 × 10 3 c , 8. 45 mg. L l ;erythromycin:k=0. 03108 8.4657×10 4 c , 14. 45 mg· L 1 . As a result of the microcalorimetry experiments, it not only indicated that antibacterial activity of clarithromycin was stronger than that of erythromycin, but also reported the changeable features of thermodynamics of the bacterial cell in biological,biochemical and metabolic process under different drug action. By using LKB 2277 Bioactivity Monitoring System, the heat effect changes in the process of inhibitory action of clarithromycin and erythromycin on Escherichia coli at 37 ° C were quantified. Quantitative analysis showed that relationship between antibiotic concentration c and rate constant k of Escherichia coli growth, and half inhibitory ratio concentration IC 50: clarithromycin: k = 0. 03003 1.1736 × 10 3 c, 8. 45 mg. L 1; erythromycin: k = 0.03108 8.4657 × 10 4 c, 14. 45 mg · L 1 As a result of the microcalorimetry experiments, it not only that that antibacterial activity of clarithromycin was stronger than that of erythromycin, but also reported the changeable features of thermodynamics of the bacterial cell in biological, biochemical and metabolic process under different drug action.
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