论文部分内容阅读
【目的】对基地和市售岗梅商品药材进行质量调查和评价。【方法】参照2010年版《中华人民共和国药典》附录ⅨH、ⅩA方法检测19批商品药材的醇溶性浸出物,采用《广东省中药材标准》中岗梅项下方法对药材进行显微鉴别,并优化薄层鉴别,建立岗梅指纹图谱。指纹图谱采用Waters XBridge TM C18色谱柱(250 mm×4.6 mm,5μm),以乙腈—体积分数0.2%磷酸梯度洗脱,流速1.0 m L/min,检测波长220 nm。【结果】样品性状、显微及薄层鉴别结果显示:收集的样品为正品,但有根茎混用现象;高效液相色谱(HPLC)指纹图谱分析结果通过相似度分析软件确定了7个共有色谱峰,检测的15批基地药材相似度均达到0.9以上,19批商品岗梅药材中有11批相似度达到0.9以上;样品醇溶性浸出物范围为64.55~186.18mg/g。【结论】收集的商品药材均为正品,但根茎混用以及生长年限不够等现象导致质量参差不齐,基地药材质量较好;本研究建立的质量评价方法可为岗梅根药材的质量评价与质量控制提供参考依据。
【Objective】 To investigate and evaluate the quality of medicinal products from base and commercial Gangmei. 【Method】 The alcohol-soluble extracts of 19 batches of medicinal herbs were tested with reference to Appendix ⅨH and ⅩA of the Pharmacopoeia of the People’s Republic of China in 2010 edition. The medicinal materials were identified by the method of Zhong Gang Mei in “Guangdong Traditional Chinese Medicine Standard” Optimize the thin layer identification, establish Gangmei fingerprinting. The fingerprint was eluted with a 0.2% phosphoric acid gradient on a Waters XBridge C18 column (250 mm × 4.6 mm, 5 μm) at a flow rate of 1.0 mL / min and a detection wavelength of 220 nm. 【Result】 The results of microscopic and TLC identification showed that the collected samples were genuine but mixed with rhizomes. The results of HPLC fingerprinting analysis confirmed seven common peaks by similarity analysis software , The similarity of all the 15 batches of medicinal herbs tested reached 0.9 or above. Among 11 batches of 19 batches of medicinal products, the similarities of 11 batches reached more than 0.9. The range of alcohol-soluble extracts was 64.55-186.18 mg / g. 【Conclusion】 The collected medicinal herbs are all genuine, but the quality of medicinal materials in the base is better because of the mixed use of rhizomes and the lack of growth life. The quality evaluation methods established in this study may be the quality evaluation and quality Control provides a reference.