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以来自我国10个省(市)、自治区的540份甘蔗细茎野生种无性系为材料,根据采集地信息及其在20对SSR引物上的分子标记数据和15个表型性状资料,开展核心种质构建研究。不同取样量(5%、10%、20%、30%、40%、50%、60%、70%、80%和90%)分析表明,10%的取样比例可获得70%以上的变异保留比例,是较好的核心种质取样规模;对5种采集地分组取样策略(等量法、简单比例法、平方根比例法、对数比例法和多样性比例法)和2种无分组取样策略(最大变异保留法和随机抽样法)比较表明,简单比例法获得的核心种质代表性最好,为最优取样策略。最后,在简单比例法取样筛选出的54份核心样品中,又通过定向选择补充了6份具有优异表型性状的材料,构建了含60份无性系的甘蔗细茎野生种核心种质,分子和表型检验都表明本研究所构建的核心种质具有较好的代表性和遗传多样性。
A total of 540 sugarcane stem-stem wild clones from 10 provinces (municipalities) and autonomous regions in China were used as materials. Based on the information collected and their molecular marker data and 15 phenotypic traits on 20 SSR primers, Germplasm construction research. Analysis of different sample volumes (5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90%) showed that more than 70% The sampling rate of core collection was the best among the five sampling sites. The sampling strategies of five sampling sites (isometric method, simple proportion method, square root proportion method, logarithmic proportion method and diversity proportion method) and two sampling strategies (Maximum Mutation Retention Method and Random Sampling Method) showed that the core collection obtained by the simple ratio method was the best, which was the optimal sampling strategy. Finally, of the 54 core samples selected by the simple proportional sampling method, six additional materials with excellent phenotypic traits were supplemented by directional selection to construct a core collection of sugarcane stems with 60 clones, And phenotypic tests all showed that the core collection constructed in this study has good representativeness and genetic diversity.