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目的:探讨儿茶酚邻位甲基转移酶(Comt)基因在小鼠动情周期中的表达调控规律,为其参与调节生殖过程的作用机制提供新的证据。方法:采用RT-PCR和实时定量PCR的方法检测成年小鼠动情周期中不同时相以及孕d7的子宫、卵巢组织Comt mRNA水平变化;性成熟前小鼠分别用E2(苯甲酸雌二醇)、P4(孕酮)、E2+Tam(苯甲酸雌二醇+他莫昔芬)、P4+RU486(孕酮+米非司酮)处理,检测其子宫和卵巢组织的mRNA水平。结果:成年小鼠动情期子宫、卵巢组织ComtmRNA水平较高,与动情前期、动情后期、动情间期相比,差异有统计学意义(P<0.05);虽然性成熟前小鼠子宫组织的各处理组与对照组间无显著性差异(P>0.05),但卵巢组织的处理组与对照组间有统计学差异(P<0.05)。卵巢组织中Comt mRNA水平E2处理组显著低于对照组,而E2+Tam组显著高于E2处理组(P<0.05)。妊娠组子宫中Comt mRNA水平显著低于未孕组(P<0.05),而卵巢组织的组间差异不显著(P>0.05)。结论:小鼠Comt mRNA水平随动情周期变化,且与妊娠有关;雌激素对Comt的生物合成具有负调节作用,而且这种作用可能是通过雌激素受体依赖性方式进行的;但是孕激素的作用不显著。
OBJECTIVE: To investigate the expression regulation of catechol ortho-methyltransferase (COMt) gene in mouse estrus cycle and to provide new evidence for its role in the regulation of reproductive process. Methods: The levels of Comt mRNA in uterus and ovary of adult mice during estrous cycle and pregnancy d7 were detected by RT-PCR and real-time quantitative polymerase chain reaction (RT-PCR). The pre-mature mice were treated with E2 (estradiol benzoate) , P4 (progesterone), E2 + Tam (estradiol benzoate + tamoxifen) and P4 + RU486 (progesterone + mifepristone). The mRNA levels of uterus and ovary were detected. Results: The levels of Comt mRNA in uterus and ovary of estrus were higher in adult mice than in estrus, estrus and estrus (P <0.05). Although the expression levels of Comt mRNA in uterus and ovary There was no significant difference between the treatment group and the control group (P> 0.05), but there was a significant difference between the treatment group and the control group (P <0.05). The level of Comt mRNA in ovarian tissue in E2 group was significantly lower than that in control group, while that in E2 + Tam group was significantly higher than that in E2 group (P <0.05). The levels of Comt mRNA in the pregnant group were significantly lower than those in the non-pregnant group (P <0.05), while there was no significant difference between the two groups (P> 0.05). CONCLUSIONS: Mouse Comt mRNA levels vary with estrous cycle and are associated with pregnancy; estrogen has a negative regulatory effect on Comt biosynthesis, and this effect may be mediated by estrogen receptor-dependent manner; however, progestin The effect is not significant.