论文部分内容阅读
目的检测外周血单个核细胞表面(PBMC)HLA-A和HLA-B在结直肠肿瘤患者中的表达,并探讨其在肿瘤发生发展中的作用。方法以Beta-actin为内参照,实时荧光定量RT-PCR方法检测结直肠肿瘤患者(结直肠肿瘤组,n=50)、结直肠良性病变患者(良性病变组,n=35)和健康体检者(正常对照组,n=42)外周血单个核细胞HLA-A和HLA-B mRNA的表达,并比较结直肠肿瘤不同TNM分期中HLA-A和HLA-B mRNA的表达。三组平均年龄相近。用Rest(relative expression software tool)软件计算相对表达率(Ratio)。结果正常对照组PBMC的HLA-A和HLA-B mRNA相对表达率分别为0.99±0.47,1.13±0.60;结直肠良性病变组相对表达率分别为0.76±0.59,0.84±0.57;结直肠肿瘤组分别为0.38±0.31和0.39±0.35。结直肠肿瘤组PBMC的HLA-A mRNA和HLA-B mR-NA相对表达率低于正常对照组和结直肠良性病变组(P<0.001)。结直肠肿瘤组HLA-A mRNA在TNM不同分期之间的表达无统计学差异(P>0.05);HLA-B mRNA在TNMⅠ期患者中的表达高于Ⅱ期和Ⅳ期(P<0.05),在其他不同分期之间差异无统计学意义(P>0.05)。结论结直肠肿瘤患者外周血单个核细胞HLA-A和HLA-B与其细胞免疫相关,在抗肿瘤免疫中起重要作用。
Objective To detect the expression of HLA-A and HLA-B on the peripheral blood mononuclear cell surface (PBMC) in patients with colorectal neoplasms and to explore its role in tumorigenesis. Methods Colorectal cancer patients (colorectal cancer group, n = 50), colorectal benign lesions (benign lesions group, n = 35) and healthy subjects were detected by using Beta-actin as internal reference and real-time fluorescence quantitative RT- (Normal control group, n = 42), and to compare the expression of HLA-A and HLA-B mRNA in different TNM staging of colorectal neoplasms. The average age of three groups is similar. The Relative Expression Software tool was used to calculate the relative expression ratio. Results The relative expression rates of HLA-A and HLA-B mRNA in PBMC of normal control group were 0.99 ± 0.47 and 1.13 ± 0.60, respectively. The relative expression rates of HLA-A and HLA-B mRNA in colorectal benign lesions were 0.76 ± 0.59 and 0.84 ± 0.57 respectively. 0.38 ± 0.31 and 0.39 ± 0.35. The relative expression of HLA-A mRNA and HLA-B mR-NA in colorectal tumor was lower than that in normal control and colorectal benign lesions (P <0.001). The expression of HLA-A mRNA in colorectal neoplasms was not significantly different between TNM stages (P> 0.05). The expression of HLA-B mRNA in TNM stage I patients was higher than that in stages II and IV (P <0.05) There was no significant difference between the different stages (P> 0.05). Conclusion Colorectal cancer patients with peripheral blood mononuclear cells HLA-A and HLA-B and its cellular immunity related to play an important role in anti-tumor immunity.