论文部分内容阅读
目的:探讨醛糖还原酶(AR)基因启动区C(-106)T多态性与糖尿病视网膜病变(DR)的关系。方法:235例江苏汉族人群,其中2型糖尿病无视网膜病变组(NDR)63例,2型糖尿病伴视网膜病变组(DR)82例,正常对照组(NC)90例,用PCR-RFLP方法检测AR基因C(-106)T基因型,比较各组等位基因及基因型分布频率。结果:未发现NDR组和NC组之间AR基因C(-106)T各等位基因及基因型频率有显著差异(P分别为0.4505,0.7279);DR组中CT及TT基因型频率均高于NC组,CC基因型频率低于NC组(P=0.0239),DR组T等位基因频率显著高于NC组,C等位基因频率显著低于NC组(P=0.0038)。结论:AR基因启动区C(-106)T多态性与江苏汉族人群DR相关,T等位基因可能是DR的遗传危险因子。
Objective: To investigate the relationship between the polymorphism of C (-106) T promoter region and diabetic retinopathy (DR) in aldose reductase (AR) gene promoter region. Methods: A total of 235 Chinese Han population of Jiangsu were recruited. Among them, 63 were type 2 diabetic patients without retinopathy (DR), 82 were type 2 diabetic patients with retinopathy (DR) and 90 were normal controls (NC). PCR-RFLP AR gene C (-106) T genotypes, alleles and genotype distribution frequency of each group were compared. Results: There was no significant difference (P = 0.4505,0.7279) between C allele and genotype of AR gene in NDR group and NC group respectively. The frequencies of CT and TT genotypes in DR group were significantly higher than those in NC group In NC group, the frequency of CC genotype was lower than NC group (P = 0.0239). The frequency of T allele in DR group was significantly higher than that in NC group. The frequency of C allele was significantly lower in NC group than in NC group (P = 0.0038). CONCLUSION: C (-106) T polymorphism in AR promoter region is associated with DR in Jiangsu Han population. T allele may be a genetic risk factor for DR.