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通过纳米金双层膜将抗风疹血清共价固定在铂电极上,从而制成高灵敏电位型风疹疫苗免疫传感器.通过抗原-抗体反应前后的电位差值考察了电极表面的电化学特性,并对该免疫传感器的性能进行了详细的研究.该传感器对风疹疫苗的检测的线性范围是3.6×10-8~1.0×10-6g/mL,检出限为1.8×10-8g/mL,线性相关系数为0.996 1.
The anti-rubella serum was covalently immobilized on the platinum electrode through a nano-gold bilayer membrane to produce a highly sensitive potentiometric rubella vaccine immunosensor.The electrochemical characteristics of the electrode surface were investigated by potentiometric difference before and after antigen-antibody reaction The performance of the immunosensor was studied in detail.The linear range of the sensor for the detection of rubella vaccine is 3.6 × 10-8 ~ 1.0 × 10-6g / mL, the detection limit is 1.8 × 10-8g / mL, the linearity The correlation coefficient is 0.996 1.