论文部分内容阅读
鉴于鸦胆丁(bruceantin)和其它苦木素化合物具有破坏核糖体的作用,引起不可逆的抑制蛋白质合成,因而以抑制[~3H]次黄嘌呤(~3H]hyp)的掺入作为评价疟原虫活力指数的方法,观察了苦木素类衍生物与真核细胞的蛋白质合成抑制剂的体外抗恶性疟原虫的作用。所用的疟原虫为T9-96株(恶性疟原虫氯喹敏感株)和K1株(恶性疟原虫抗氯喹和抗乙胺嘧啶株)。2种原虫均培养在悬浮于RPMI 1640的人A~+红细胞中,培养液中加有D-葡萄糖和10％人A~+血清。选择富含环状体的疟原虫作药敏试验,即将T9-96或
Since bruceantin and other bitter lignin compounds have an effect of damaging the ribosomes and cause irreversible inhibition of protein synthesis, incorporation of [-3H] -hypoxanthin (~ 3H) hyp) Viability index, the effect of bitternin derivatives against the P. falciparum in vitro against protein synthesis inhibitors of eukaryotes was observed. The plasmids used are T9-96 (Plasmodium falciparum chloroquine sensitive) and K1 (Plasmodium falciparum chloroquine and anti-pyrimethamine). Both species were cultured in human A ~ + erythrocytes suspended in RPMI 1640 supplemented with D-glucose and 10% human A ~ + serum. Select the ring-rich malaria parasite for drug susceptibility testing, about T9-96 or