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目的:探讨盐酸千金藤碱(CH)对小鼠急性脂肪肝的治疗作用及机制。方法:腹腔注射地塞米松(DXM)的同时灌服高脂饮食复制小鼠急性脂肪肝模型,应用不同剂量CH对其进行治疗,观察小鼠血脂及肝脂、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)等指标及肝脏病理学的影响。结果:与正常组相比,模型组小鼠血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性和肝组织TG、TC、MDA含量显著升高(P<0.01),肝组织SOD和GSH-Px活性显著降低(P<0.01)。CH高剂量组小鼠血清TG、TC、ALT、AST及肝组织TG、TC、MDA显著低于模型组(P<0.01,P<0.05),肝组织SOD和GSH-Px活性显著高于模型组(P<0.01);不同剂量CH均能改善肝细胞脂肪变性情况。结论:CH对DXM所致小鼠急性脂肪肝具有良好的治疗作用,其机制可能与其能够提高抗氧化酶活性、抑制脂质过氧化反应等方面有关。
Objective: To investigate the therapeutic effect and mechanism of chalcone hydrochloride (CH) on acute fatty liver in mice. METHODS: Acute fatty liver models of mice were induced by intraperitoneal injection of dexamethasone (DXM) and fed with a high-fat diet. Different doses of CH were used to treat them. Blood lipids, hepatic lipids, and superoxide dismutase (SOD) were observed. , glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and other indicators and liver pathological effects. Results: Compared with the normal group, the mice in the model group had serum triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and liver tissue TG, TC, The MDA content was significantly increased (P<0.01), and the activity of SOD and GSH-Px in liver tissue was significantly decreased (P<0.01). The levels of TG, TC, ALT, AST and TG, TC, MDA in liver tissue were significantly lower in CH group than in model group (P<0.01, P<0.05). The activities of SOD and GSH-Px in liver tissue were significantly higher than those in model group. (P<0.01); Different doses of CH all improved hepatocyte steatosis. Conclusion: CH has a good therapeutic effect on DXM-induced acute fatty liver in mice. Its mechanism may be related to its ability to increase antioxidant enzyme activity and inhibit lipid peroxidation.