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目的:研究养阴、益气活血方药及其组合抗凝、纤溶及抗自由基损伤的作用。方法:采用血清药理学方法研究养阴、益气活血方药及其组合对培养人脐静脉内皮细胞抗凝、纤溶及抗自由基损伤的作用,并对其作用进行对比分析。结果:养阴方具有显著提高前列环素(6-keto-PGF_(1α))含量;提高组织型纤溶酶原激活物(t-PA)活性,抑制组织型纤溶酶原激活抑制物(PAI)活性;提高超氧化物歧化酶(SOD)活力,降低丙二醛(MDA)含量;降低内皮素(ET)含量,提高一氧化氮(NO)含量等作用。结论:养阴方药与益气活血方药相伍在抗栓、溶栓、抗自由基损伤等方面具有协同作用,认为养阴生津是治疗血瘀证的重要法则。
Objective: To study the effects of Yangyin, Qi-invigorating and blood-activating herbs and their combination anticoagulation, fibrinolysis and anti-free radical injury. METHODS: Serum pharmacological methods were used to study the effects of Yangyin, Yiqi Huoxue Recipe and their combination on the anticoagulant, fibrinolysis and anti-free radical damage of cultured human umbilical vein endothelial cells, and their effects were compared. RESULTS: Yangyin Decoction significantly increased the content of prostacyclin (6-keto-PGF_(1α)); increased the activity of tissue-type plasminogen activator (t-PA) and inhibited tissue-type plasminogen activator inhibitor ( PAI) activity; increase the activity of superoxide dismutase (SOD), decrease the content of malondialdehyde (MDA); decrease the content of endothelin (ET) and increase the content of nitric oxide (NO). Conclusion: The combination of Yangyin Decoction and Yiqi Huoxue Decoction has a synergistic effect on antithrombotic, thrombolytic and anti-radical damage. It is considered that Yangyin Shengjin is an important rule for the treatment of blood stasis syndrome.